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小鼠6酮前列腺素F1a酶免試劑盒,(6-keto-PGF1a)ELISA檢測試劑盒 ,英文名:6-keto-PGF1a ELISA Kit ,英文縮寫:6-keto-PGF1a ,常見試劑盒規(guī)格:96T/48T,產品別名:小鼠6酮前列腺素F1aELISA檢測試劑盒,6-keto-PGF1a 檢測試劑盒, 6-keto-PGF1a 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠6酮前列腺素F1a酶免試劑盒,(6-keto-PGF1a)ELISA檢測試劑盒在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發(fā)生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質。可用洗板機洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
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Aristaless樣同源框蛋白4(ALX4)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Aristaless Like Homeobox Protein 4 (ALX4)96TY17547Rattus norvegicus (Rat)
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成纖維細胞生長因子4(FGF4)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 4 (FGF4)96TY17558Bos taurus; Bovine (Cattle)
成纖維細胞生長因子4(FGF4)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 4 (FGF4)96TY17559Chicken (Gallus)
成纖維細胞生長因子4(FGF4)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 4 (FGF4)96TY17560Mus musculus (Mouse)
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成纖維細胞生長因子6(FGF6)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 6 (FGF6)96TY17564Mus musculus (Mouse)
成纖維細胞生長因子6(FGF6)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 6 (FGF6)96TY17565Rattus norvegicus (Rat)
成纖維細胞生長因子9(FGF9)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 9 (FGF9)96TY17566Bos taurus; Bovine (Cattle)
成纖維細胞生長因子9(FGF9)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 9 (FGF9)96TY17567Chicken (Gallus)
成纖維細胞生長因子9(FGF9)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 9 (FGF9)96TY17568Homo sapiens (Human)
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FMS樣酪氨酸激酶3配體(Flt3L)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for FMS Like Tyrosine Kinase 3 Ligand (Flt3L)96TY17571Homo sapiens (Human)
FMS樣酪氨酸激酶3配體(Flt3L)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for FMS Like Tyrosine Kinase 3 Ligand (Flt3L)96TY17572Mus musculus (Mouse)
中性粒細胞激活蛋白3(NAP3)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Neutrophil Activating Protein 3 (NAP3)96TY17573Homo sapiens (Human)
中性粒細胞激活蛋白3(NAP3)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Neutrophil Activating Protein 3 (NAP3)96TY17574Mus musculus (Mouse)
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白介素3調節(jié)核因子(NFIL3)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Nuclear Factor, Interleukin 3 Regulated (NFIL3)96TY17576Homo sapiens (Human)
白介素3調節(jié)核因子(NFIL3)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Nuclear Factor, Interleukin 3 Regulated (NFIL3)96TY17577Mus musculus (Mouse)
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PDGFA關聯(lián)蛋白1(PDAP1)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for PDGFA Associated Protein 1 (PDAP1)96TY17579Homo sapiens (Human)
PDGFA關聯(lián)蛋白1(PDAP1)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for PDGFA Associated Protein 1 (PDAP1)96TY17580Mus musculus (Mouse)
PDGFA關聯(lián)蛋白1(PDAP1)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for PDGFA Associated Protein 1 (PDAP1)96TY17581Rattus norvegicus (Rat)
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正輔因子4(PC4)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Positive Cofactor 4 (PC4)96TY17585Homo sapiens (Human)
正輔因子4(PC4)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Positive Cofactor 4 (PC4)96TY17586Mus musculus (Mouse)
正輔因子4(PC4)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Positive Cofactor 4 (PC4)96TY17587Rattus norvegicus (Rat)
胰島素生長因子樣家族成員2(IGFL2)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for IGF Like Family, Member 2 (IGFL2)96TY17588Homo sapiens (Human)
胰島素生長因子樣家族成員3(IGFL3)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for IGF Like Family, Member 3 (IGFL3)96TY17589Homo sapiens (Human)
胰島素生長因子樣家族成員3(IGFL3)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for IGF Like Family, Member 3 (IGFL3)96TY17590Mus musculus (Mouse)
胰島素生長因子樣家族成員3(IGFL3)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for IGF Like Family, Member 3 (IGFL3)96TY17591Rattus norvegicus (Rat)
胰島素生長因子樣家族成員4(IGFL4)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for IGF Like Family, Member 4 (IGFL4)96TY17592Homo sapiens (Human)
胰島素生長因子樣家族成員1(IGFL1)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for IGF Like Family, Member 1 (IGFL1)96TY17593Homo sapiens (Human)
趨化素樣因子超家族成員8(CKLFSF8)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Chemokine Like Factor Superfamily 8 (CKLFSF8)96TY17594Homo sapiens (Human)
趨化素樣因子超家族成員8(CKLFSF8)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Chemokine Like Factor Superfamily 8 (CKLFSF8)96TY17595Mus musculus (Mouse)
各種類型ELISA測定時,一般需經過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。