服務(wù)熱線
021-54479081
021-54461587
產(chǎn)品展示PRODUCTS
本產(chǎn)品僅用于科研,不用于臨床
小鼠單核細(xì)胞趨化蛋白4酶免試劑盒,(MCP-4/CCL13)ELISA檢測試劑盒 ,英文名:MCP-4/CCL13 ELISA Kit ,英文縮寫:MCP-4/CCL13 ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠單核細(xì)胞趨化蛋白4ELISA檢測試劑盒,MCP-4/CCL13 檢測試劑盒, MCP-4/CCL13 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠單核細(xì)胞趨化蛋白4酶免試劑盒,(MCP-4/CCL13)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
外周鞘磷脂蛋白22(PMP22)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Peripheral Myelin Protein 22 (PMP22)48T/96TY16785Homo sapiens (Human)
肽聚糖識別蛋白2(PGLYRP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Peptidoglycan Recognition protein 2 (PGLYRP2)48T/96TY16786Homo sapiens (Human)
ATP結(jié)合盒轉(zhuǎn)運蛋白A4(ABCA4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for ATP Binding Cassette Transporter A4 (ABCA4)48T/96TY16787Homo sapiens (Human)
含STIP1同源物U-框蛋白1(STUB1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for STIP1 Homology And U-Box Containing Protein 1 (STUB1)48T/96TY16788Mus musculus (Mouse)
PDZ和LIM域蛋白1(PDLIM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for PDZ And LIM Domain Protein 1 (PDLIM1)48T/96TY16789Homo sapiens (Human)
成神經(jīng)細(xì)胞瘤抑制瘤變蛋白1(NBL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neuroblastoma, Suppression Of Tumorigenicity 1 (NBL1)48T/96TY16790Homo sapiens (Human)
生長停滯特異性蛋白6(GAS6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Growth Arrest Specific Protein 6 (GAS6)48T/96TY16791Rattus norvegicus (Rat)
CD14分子(CD14)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cluster Of Differentiation 14 (CD14)48T/96TY16792Mus musculus (Mouse)
肝素酶(HPA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heparanase (HPA)48T/96TY16793Rattus norvegicus (Rat)
細(xì)胞程序性死亡蛋白1配體2(PDCD1LG2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Programmed Cell Death Protein 1 Ligand 2 (PDCD1LG2)48T/96TY16794Homo sapiens (Human)
腫瘤壞死因子配體超家族成員14(TNFSF14)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14)48T/96TY16795Homo sapiens (Human)
三葉因子1(TFF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Trefoil Factor 1 (TFF1)48T/96TY16796Rattus norvegicus (Rat)
腫瘤壞死因子受體超家族成員5(TNFRSF5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Receptor Superfamily, Member 5 (TNFRSF5)48T/96TY16797Rattus norvegicus (Rat)
轉(zhuǎn)錄激活因子6(ATF6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Activating Transcription Factor 6 (ATF6)48T/96TY16798Mus musculus (Mouse)
Thy1細(xì)胞表面抗原(Thy1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Thy1 Cell Surface Antigen (Thy1)48T/96TY16799Homo sapiens (Human)
粒細(xì)胞集落刺激因子受體(GCSFR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Colony Stimulating Factor Receptor, Granulocyte (GCSFR)48T/96TY16800Homo sapiens (Human)
白介素17受體A(IL17RA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 17 Receptor A (IL17RA)48T/96TY16801Homo sapiens (Human)
CD163分子(CD163)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cluster Of Differentiation (CD163)48T/96TY16802Mus musculus (Mouse)
Salusinβ蛋白(Salusinβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Salusin Beta48T/96TY16803Rattus norvegicus (Rat)
Ⅱ類主要組織相容性復(fù)合體DQβ1(MHCDQβ1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Major Histocompatibility Complex Class II DQ Beta 1 (MHCDQb1)48T/96TY16804Homo sapiens (Human)
非受體型蛋白酪氨酸磷酸酶1(PTPN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Tyrosine Phosphatase, Non Receptor Type 1 (PTPN1)48T/96TY16805Homo sapiens (Human)
拓?fù)洚悩?gòu)酶Ⅰ(*)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Topoisomerase I (*)48T/96TY16806Mus musculus (Mouse)
成纖維細(xì)胞生長因子21(FGF21)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 21 (FGF21)48T/96TY16807Rattus norvegicus (Rat)
對氧磷酶2(PON2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Paraoxonase 2 (PON2)48T/96TY16808Mus musculus (Mouse)
核連蛋白1(NUCB1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nucleobindin 1 (NUCB1)48T/96TY16809Rattus norvegicus (Rat)
核連蛋白2(NUCB2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nucleobindin 2 (NUCB2)48T/96TY16810Rattus norvegicus (Rat)
嘌呤霉素敏感性氨肽酶(PSA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Puromycin Sensitive Aminopeptidase (PSA)48T/96TY16811Homo sapiens (Human)
WAP四二硫化物核心域蛋白5(WFDC5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for WAP Four Disulfide Core Domain Protein 5 (WFDC5)48T/96TY16812Homo sapiens (Human)
真核翻譯起始因子2α激酶3(EIF2αK3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3)48T/96TY16813Mus musculus (Mouse)
肉毒堿棕櫚酰基轉(zhuǎn)移酶1A(CPT1A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Carnitine Palmitoyltransferase 1A, Liver (CPT1A)48T/96TY16814Rattus norvegicus (Rat)
氧固醇結(jié)合蛋白樣蛋白8(OSBPL8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Oxysterol Binding Protein Like Protein 8 (OSBPL8)48T/96TY16815Homo sapiens (Human)
核苷酸還原酶M1(RRM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ribonucleotide Reductase M1 (RRM1)48T/96TY16816Mus musculus (Mouse)
銜接因子相關(guān)蛋白復(fù)合體2μ1(AP2μ1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adaptor Related Protein Complex 2 Mu 1 (AP2m1)48T/96TY16817Homo sapiens (Human)
5-甲基四氫葉酸高半胱氨酸甲基轉(zhuǎn)移酶(MTR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for 5-Methyltetrahydrofolate Homocysteine Methyltransferase (MTR)48T/96TY16818Homo sapiens (Human)
α-生育酚轉(zhuǎn)運蛋白(TTPα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alpha Tocopherol Transfer Protein (TTPa)48T/96TY16819Mus musculus (Mouse)
前列腺轉(zhuǎn)谷氨酰胺酶(TGM4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transglutaminase 4, Prostate (TGM4)48T/96TY16820Homo sapiens (Human)
維甲酸受體應(yīng)答基因1(RARRES1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Retinoic Acid Receptor Responder 1 (RARRES1)48T/96TY16821Homo sapiens (Human)
蘋果酸脫氫酶1(MDH1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Malate Dehydrogenase 1 (MDH1)48T/96TY16822Rattus norvegicus (Rat)
DNA甲基轉(zhuǎn)移酶1(DNMT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for DNA Methyltransferase 1 (DNMT1)48T/96TY16823Mus musculus (Mouse)
DNA損傷誘導(dǎo)轉(zhuǎn)錄因子3(DDIT3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for DNA Damage Inducible Transcript 3 (DDIT3)48T/96TY16824Mus musculus (Mouse)
四加半LIM域蛋白1(FHL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Four And A Half LIM Domains Protein 1 (FHL1)48T/96TY16825Homo sapiens (Human)
前膠原賴氨酸-2-酮戊二酸-5-雙加氧酶1(PLOD3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Procollagen Lysine-2-Oxoglutarate-5-Dioxygenase 3 (PLOD3)48T/96TY16826Homo sapiens (Human)
角蛋白1(KRT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Keratin 1 (KRT1)48T/96TY16827Rattus norvegicus (Rat)
α白蛋白(AFM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Afamin (AFM)48T/96TY16828Rattus norvegicus (Rat)
白介素8受體β(IL8Rβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 8 Receptor Beta (IL8Rb)48T/96TY16829Mus musculus (Mouse)
巖藻糖基轉(zhuǎn)移酶8(FUT8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fucosyltransferase 8 (FUT8)48T/96TY16830Mus musculus (Mouse)
溶質(zhì)載體家族16成員1(SLC16A1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Solute Carrier Family 16, Member 1 (SLC16A1)48T/96TY16831Homo sapiens (Human)
趨化因子C-C-基元受體5(CCR5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Chemokine C-C-Motif Receptor 5 (CCR5)48T/96TY16832Homo sapiens (Human)
Canopy 2同源物(CNPY2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Canopy 2 Homolog (CNPY2)48T/96TY16833Homo sapiens (Human)
轉(zhuǎn)酮醇酶(TKT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transketolase (TKT)48T/96TY16834Rattus norvegicus (Rat)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。